Adapting Drosophila melanogaster Cell Lines to Serum-Free Culture Conditions
نویسندگان
چکیده
منابع مشابه
Cell lines derived from late embryonic stages of Drosophila melanogaster.
The development of three cell lines initiated from the late embryonic stages of Drosophila melanogaster is described. The primary cultures consisted of trypsinized fragments from embryos 20-24 h old. The length of time between primary culture and subsequent subculture varied from 8 months for the first line to 3 weeks for the third. All three lines have been maintained in vitro for more than a ...
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A procedure is described for preparing cell-free protein synthesizing lysates from Drosophila melanogaster tissue culture cells and embryos. Preparation of translationally active lysates from tissue culture cells is dependent on the presence of rat liver supernatant during cell lysis to inhibit ribonuclease activity. After micrococcal nuclease treatment of the lysate, protein synthesis is depen...
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Thymic epithelial cells (TEC) have been cultured for several months and/or for 4 to 5 transfers in a growth factor-defined serum-free medium without concurrent growth of other cell types. The use of monoclonal antibodies and alpha MAM-6 indicated that the majority of TEC were of medullary origin. The vast majority of cells were positive for LFA-3 and class I, and class II expression was low or ...
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The proliferation of pancreatic duct-like CK19+ cells has implications for multiple disease states including pancreatic cancer and diabetes mellitus. The in vitro study of this important cell type has been hampered by their limited expansion compared to fibroblast-like vimentin+ cells that overgrow primary cultures. We aimed to develop a screening platform for duct cell mitogens after depletion...
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Many motile organisms localize the source of attractive odorants by following plumes upwind. In the case of D. melanogaster, little is known of how individuals alter their flight trajectories after encountering and losing a plume of an attractive odorant. We have characterized the three-dimensional flight behavior of D. melanogaster in a wind tunnel under a variety of odor conditions. In the ab...
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ژورنال
عنوان ژورنال: G3 Genes|Genomes|Genetics
سال: 2020
ISSN: 2160-1836
DOI: 10.1534/g3.120.401769